Application

The unconjugated antibody (Cat. No. 06-680) is shown to be suitable also for chromatin immunoprecipitation (ChIP), ChIP-seq, Western blotting, immunoprecipitation, flow cytometry, immunohistochemistry applications.

Research Sub CategoryHormones & Receptors

Research CategoryEpigenetics & Nuclear Function

This Anti-Androgen Receptor Antibody, Alexa Fluor^® 555 Conjugate is validated for use in Immunocytochemistry for the detection of Androgen Receptor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Androgen receptor (UniProt P15207; also known as Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4) is encoded by the Ar (also known as Andr, Nr3c4, Tfm) gene (Gene ID 24208) in rat species. Androgen receptor (AR) is a hormone-activated transcription regulator that plays important roles in normal prostate development and function, as well as in malignant prostate transformation. The binding of an androgenic hormone (testosterone or dihydrotestosterone) induces a conformational change in AR, resulting in its dissociation from heat schock proteins and subsequent translocation from the cytosol to nucleus, where it dimerizes and mediates either transcription activation or repression in a target gene-specific manner. The androgen insensitivity syndrome (AIS) or testicular feminization (TFM) is caused by a mutation of the androgen receptor gene located on the X chromosome.

Immunogen

Epitope: N-terminal modulating region.

KLH-conjugated linear peptide corresponding to the N-terminal sequence of rat androgen receptor.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Affinity purified.

Quality

Evaluated by Immunocytochemistry in T47D cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected androgen receptor in T47D human breast cancer cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~110 kDa observed.